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BECKMAN COULTER

Flow Cytometry Reagent - PerFix-P-nc (no centrifugation ) - Cell Preparation

An innovative tool for flow cytometric analysis of multiple intracellular epitopes, or both multiple intracellular and cell ...
QUOTATION
INFORMATION
QUOTATION
INFORMATION
DESCRIPTION LITERATURES SPECIFICATION APPLICATION NOTES LINK
DESCRIPTION
Simplify the workload necessary for the sample preparation.

Perfix-nc dramatically reduces the number of manipulative steps
• Less than 15 minutes. workload hands-on-time
• Fastest intracellular staining method
• No centrifuge, no washing steps with STRAIGHT procedure PerFix-nc Workflow and Workload


Achieve accurate detection of both intracellular and extracellular epitopes
• Excellent reproducibility
• Minimizes cell loss and aggregate formation
• Preserves scatter characteristics and MFIs

Several surface markers can be added together with the intracellular markers and incubated simultaneously
• Adaptable to specific antigen detection requirements
• Amazing cell recovery and gate purity
• Enhanced signal-to-noise ratio of intracellular staining

Surface & intracellular staining in a single step
• Optimized for human whole blood and human bone marrow
• Can also be used on mouse blood
• Automation ready

Excellent preservation of epitopesPerFix-nc Workflow and Workload

Cytokine StainingPerFix-nc Cytokine Staining


Part of our complete line of preparation kits
PerFix-nc preparation kits


The PerFix-nc Kit (no centrifuge assay Kit) has been developed to enhance the signal-to-noise ratio of intracellular staining and simplify the workload necessary for the sample preparation.

Accurate detection of both intracellular and extracellular epitopes are obtained, while:
• There are no washing steps through the whole STRAIGHT procedure (only a final wash step is described as
optional).
• Several surface markers can be added together with the intracellular markers and incubated simultaneously
during the permeabilization step.
• Total duration of the procedure and total workload are similar to current procedures for surface staining (e.g.
VersaLyse + Fix No Wash), and much shorter than common permeabilization procedures.
• Potential automation of the PerFix-nc STRAIGHT procedure is rendered possible thanks to the removal of the
washing steps.




PerFix-nc dotplot Illustration of leucocyte pattern and intracytoplasmic B-Cell staining on normal sample, implementing the PerFix-nc STRAIGHT protocol. PerFix-nc histogram ZAP-70-PE staining on lymphocytes of another normal sample, implementing the PerFix-nc + wash protocol.
LITERATURES
SPECIFICATION
APPLICATION NOTES
LINK
BECKMAN COULTER website
BECKMAN COULTER
Solutions and systems for clinical diagnostics (hematology, biochemistry, immunoassays, flow cytometry. Solutions for industry & research, for genetics, proteomics and cellular analysis. Lab automated workstation & liquid handling workstation.
This item belongs to the following categories (click to expand):
Prices are quoted in euro (taxes excluded) and apply in the Benelux (recycling fee included). They are subject to change without notice. We reserve the right to adjust pricing errors and to limit quantities.

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