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BECKMAN COULTER

CE Kits - Proteins Methods Development /CE Method Dev

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ceofix@analis.be
++(0)81 250 50 50
QUOTATION
INFORMATION
ceofix@analis.be ++(0)81 250 50 50
DESCRIPTION CE METHODS DEVELOPMENT PROTEIN METHODS DEVELOPMENT
DESCRIPTION
Protein Methods Development

With capillary zone electrophoresis (CZE) proteins are separated by their electrophoretic mobility, a function of the charge and mass of a protein at a given pH. When using electrolytes with pH > 4.0, the surface of bare-fused silica becomes negatively charged, inducing both electroosmotic flow (EOF) and the adsorption of proteins that possess a net positive charge. The Protein Methods Development kit, contains coated capillaries, buffers, standards and EOF markers to allow you to optimize a separation method for the analysis of a broad spectrum of proteins. The use of this Neutral capillary surface serves to significantly reduce EOF and to prevent adsorption of proteins to the capillary surface. These Neutral capillaries have a two layer coating, with the first layer a bonded phase serving to deactivate the silanol groups and a second hydrophilic layer that protects against hydrophobic interactions improving the overall efficiency and resolution of proteins separated by CZE. This chemistry is designed to be used with the PA 800 plus Pharmaceutical Analysis System UV detector only - the higher energy generated by the PDA detector may serve to degrade the capillary surface

CE Methods Development

The P/ACE™ MDQ Methods Development Kit is designed to give a starting point for the development of a capillary zone electrophoresis analysis of complex proteins, pharmaceuticals, or basic analytes. This kit includes both neutral (polyacrylamide-based) and amine coated capillaries. Benzyl alcohol is included as a neutral EOF marker while histamine is used as a reference marker for the neutral capillary

CE METHODS DEVELOPMENT
PROTEIN METHODS DEVELOPMENT
BECKMAN COULTER website
BECKMAN COULTER
Solutions and systems for clinical diagnostics (hematology, biochemistry, immunoassays, flow cytometry. Solutions for industry & research, for genetics, proteomics and cellular analysis. Lab automated workstation & liquid handling workstation.
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Prices are quoted in euro (taxes excluded) and apply in the Benelux (recycling fee included). They are subject to change without notice. We reserve the right to adjust pricing errors and to limit quantities.

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