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BECKMAN COULTER

CE Kits for Nucleic Acid Analysis

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ceofix@analis.be
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ceofix@analis.be ++(0)81 250 50 50
DESCRIPTION LINK
DESCRIPTION
BECKMAN COULTER
Versatile platform capable of multitude
of genetic analysis applications

GenomeLab™ GeXP Genetic Analysis System

GenomeLab™ GeXP Genetic Analysis System

P/ACE™ MDQ

P/ACE™ MDQ


Nucleic Acid Analysis  Genotyping Automation

The automation of gel loading, sample introduction,
detection and analysis saves time over
conventional genotyping processes.
Resolution is sufficient to allow 2-5 bp differences resolved
from double-stranded fragments under native electrophoresis
conditions, with run-to-run migration
time reproducibility typically less than 1.0% R.S.D.

Nucleic Acid Analysis - Genotyping Automation



How to Streamline Oligonucleotide ?

The high resolving power of capillary gel electrophoresis (CGE)
and direct UV detection allow the separation and quantitation
of oligonucleotides with single base resolution.

Illustrated is the separation of a 45-mer oligonucleotide,
with clear resolution of the n-1 product

Nucleic Acid Analysis - Oligonucleotide Purity



Nucleic Acid Analysis  - Plasmid Analysis

garose Gel Electrophoresis (AGE) has been the primary method used to assess the homogeneity of plasmid DNA, however, this approach has some major disadvantages. The AGE method is manual, only semi-quantitative and the assignment of bands to plasmid structures is difficult, as the electrophoretic mobility of plasmids of different shapes changes with the electrophoresis operating conditions A more powerful routine technology for the quantification of plasmid forms is Capillary Gel Electrophoresis (CGE). This automated approach offers high resolution, high sensitivity and high reproducibility to this analysis.

Nucleic Acid Analysis - Plasmid Analysis

Separation of pUC19 (2.7 kbp) plasmid structures, using the P/ACE™ capillary electrophoresis system
(Schmidt et al, P/ACE™ Setter Newsletter, Vol. 4 Issue 2, October 2000).


Nucleic Acid Analysis - SSCP Analysis

There are Two Kinds of Mutation Detection Methods Specific and Scanning

The former is used to identify specific, well-characterized sequence variations, while the latter one is used to detect uncharacterized sequence mutations. SSCP is an example of mutation scanning technology. Mutation detection via conventional SSCP requires PCR* amplification of the DNA fragment of interest, denaturation of the double-stranded product, followed by non-denaturing slab gel electrophoresis. The need for higher efficiency detection, greater automation and safety have led to studies using capillary electrophoresis-based SSCP analysis.

Nucleic Acid Analysis - Single-Stranded Conformational Polymorphism (SSCP) Analysis

Figures 1(a) and 1(b) illustrate examples of CE-SSCP profiles derived from both the wild type and mutant 185delAG alleles. As shown, the profiles for both the forward and reverse strands of wild type are qualitatively similar
[Fig. 1A (a) and (b)], as are those for the mutant 185delAG allele [Fig. 1B (a) and (b)].
Most important, there are consistent qualitative differences between the wild type and the mutated strands in the profiles. These differences are manifested in the form of three peaks in the SSCP profile of the wild type, where there are five peaks in the SSCP profile of the mutant allele - allowing the wild type and mutant alleles to be easily discriminated via visual inspection (Click the image to see a full-size version of the graph) (Landers et al., P/ACE™ Setter Newsletter, Vol. 3 Issue 4, December 1999).

* All trademarks are the property of their respective owners.

For Research Use Only; not for use in diagnostic procedures.



Nucleic Acid Analysis - Quantitation by Direct Hybridization

Viral Load

CE-LIF provides the sensitivity required for direct hybridization analysis in solution.
DNA-probe complexes can be detected at very low levels, removing the need for PCR* amplification.
Separation and quantitation of complexes from unbound probes is typically achieved in less than 20 minutes.

Nucleic Acid Analysis: Quantitation by Direct Hybridization

*All trademarks are the property of their respective owners.

For Research Use Only; not for use in diagnostic procedures.



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BECKMAN COULTER website
BECKMAN COULTER
Solutions and systems for clinical diagnostics (hematology, biochemistry, immunoassays, flow cytometry. Solutions for industry & research, for genetics, proteomics and cellular analysis. Lab automated workstation & liquid handling workstation.
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Prices are quoted in euro (taxes excluded) and apply in the Benelux (recycling fee included). They are subject to change without notice. We reserve the right to adjust pricing errors and to limit quantities.

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