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ANALIS

ISOPAL™ Plus Alkaline Phosphatase Isoenzyme Electrophoresis Kit

Serum alkaline phosphatase (AP) is found in almost all body tissues. It is manufactured by bone (40 to 70 percent), liver, intestine and placenta
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+32 (0) 81 25 50 50
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ceofix@analis.be +32 (0) 81 25 50 50
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DESCRIPTION
Clinical Significance

Serum alkaline phosphatase (AP) is found in almost all body tissues. It is manufactured by bone (40 to 70 percent), liver, intestine and placenta.

As an enzyme of secretion, AP is constantly and rapidly released from the cell into the interstitial area and then into the serum.


The activity of AP offers important information about bone formation (osteoblastic activity) and is clinically useful as a test of liver function. AP isoenzymes have been identified as the following four classes:

Class1 - Tissue unspecific (liver, bone, renal)
Class 2 - Intestinal

Class 3 - Placental

Class 4 - Fetal intestine

* Isopal is a trademark of Analis, S.A.



As glycoproteins, AP isoenzymes can be separated by their differential charges in an electric field, making the various fractions clearly recognizable. Therefore, electrophoresis is considered the method of choice for quantification of serum AP.

Isopal™ Plus Alkaline

Phosphatase Isoenzyme Kit

The Isopal™ Plus electrophoresis method provides distinct separation of all AP isoenzymes in one easy to use,

reproducible, high-volume procedure. The distinct patterns resulting from the Isopal™ Plus method are especially useful in differentiating bone and liver fractions when diagnosing bone and liver diseases.

Isopal's unique gel/buffer combination ensures superior resolution and sensitivity, which aids in the detection of intestinal fractions and atypical variants, while its colorimetric methodology provides a permanent, visible record

for quantification and archiving.

Additionally, the Isopal™ Plus kit offers confirmatory reagent (Neuraminidase, Anti-placental Antibody and Ficin), which enhance the differentiating process by allowing separation of bands that would otherwise co-migrate.

MSDS
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BROCHURE
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Prices are quoted in euro (taxes excluded) and apply in the Benelux (recycling fee included). They are subject to change without notice. We reserve the right to adjust pricing errors and to limit quantities.

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