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BECKMAN COULTER

SPRI® works HT-High Sample Preparation for Illumina NGS Platforms

High throughput Biomek -based solution prepares up to 96 libraries with size selection in just 6 hours or 3 hrs with no size selection. In addition to library construction, the system offers per well size selection so samples for multiple applications can be processed on the same plate.
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DESCRIPTION LINK BECKMAN & ILLUMINA DOWNLOAD BIOMEK OTHER PRODUCT
DESCRIPTION

SPRIworks HT-High Throughput Sample Preparation for Illumina NGS Platforms High throughput Biomek -based solution prepares up to 96 libraries with size selection in just 6 hours or 3 hrs with no size selection. In addition to library construction, the system offers per well size selection so samples for multiple applications can be processed on the same plate.
For added efficiency, downstream methods for quantitation, normalization and pooling are included

Sample Prep Freedom for High Throughput Next Generation Sequencing Labs

Proven SPRI chemistry is combined with the flexibility of the Beckman Coulter Biomek FXP liquid handler to revolutionize how next generation sequencing samples are prepared. All chemistry needed to prepare libraries is included in a kit formatted for high throughput processing. Also included are reagents to perform size selection, PCR setup and cleanup. The only thing left to do is add the DNA sample and adapter of choice.

Options, options, options…because SPRIworks HT is automated on the Biomek automated liquid handler, users have many options for how they process their samples.
 

Key Features:
Process up to 96 samples with size selection in less than 6hrs. 3hrs if no size selection.
SPRI- Based per well size selection enables processing samples for multiple applications in a single plate.
Quick Implementation with validated methods standardized process
Automated methods for all major steps. Run them all or pick and choose what fits in your day.
  »
Library Construction
  » Size Selection
  » PCR Setup
  » Quantitation*
  » Normalization
  » Sample pooling for multiplexing
Reproducible and consistent library output
Reduced sequencing cost/sample
Improved sequencing service turn-around time
Libraries compatible with:
  » RNA-Seq, CHiP-Seq and Target capture applications
  » Regular fragment or paired end adapters

Increase Efficiency and Throughput

  • Up to 96 high quality, fully constructed and sized libraries in under 6 hours
  • Up to 25 times the throughput of manual library construction, similar to
    having 25 additional people to do next generation sequencing sample preparation

Beckman Coulter SPRIworks vs Manual Processing Methods

 

SPRIworks HT Comparison
 
 
Simplicity and Flexibility in the Graphical User Interface
The setup process to prepare up to 96 libraries takes less than 30 minutes of hands on time.
The SPRIworks HT end-user provides input DNA and adaptors. All other reagents are included in the SPRIworks HT fragment library kit. 

Step 1. Configure parameters using the intuitive user interface
 

Configuring parameters

Step 2. Method calculates reagent volumes based on user input application reagents

SPRIworks -  reagent volumes calculation

Step 3. Place tips, plates and reagents on the instrument, click OK 
 

SPRIworks HT - GUI

 

Built-in SPRI Size Selection Chemistry and Clean-up

The SPRI chemistry has been optimized to purify fragmented DNA and size select the prepared library.

Three different size ranges are available, as well as no size selection for processing amplicons.

This feature replaces the need for gel purification and column based clean-up.

Short Fragments 150-350 bp (insert sizes)
Medium Fragments 250-450 bp (insert sizes)
Long Fragments 350-700 bp (insert sizes)
No Size Selection

 

SPRI - Short Fragments SPRI - Medium Fragments SPRI - Long Fragments


Size selection range for short (A), medium(B) and long ® fragment sizes (Agilent high sensitivity DNA chip).

SPRIworks HT Yield graph image

Resulting PCR yields in nM, as determined by qPCR, for 1 µg of
E.coli processed through the SPRIworks HT method suite. Library elution volume was 20 µL,
PCR input volume was 20 µL and was cycled 10 times.



System Specifications

Up to 96 high quality, fully constructed and sized libraries in under 6 hours.

SPRIworks HT Library Construction image 


System Components

SPRIworks HT Fragment Library Kit 48 rxn- For Illumina NGS B06938
SPRIworks HT Biomek FXP Dual Multi channel 96 and Span 8
(includes B13563, SPRIworks HT Methods CD)
B05450
SPRworks-HT Consumables Bundle FXP (96X10) B05451
SPRworks-HT Methods CD (Illumina) B13563

 

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BECKMAN & ILLUMINA
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BIOMEK
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BECKMAN COULTER website
BECKMAN COULTER
Solutions and systems for clinical diagnostics (hematology, biochemistry, immunoassays, flow cytometry. Solutions for industry & research, for genetics, proteomics and cellular analysis. Lab automated workstation & liquid handling workstation.
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Prices are quoted in euro (taxes excluded) and apply in the Benelux (recycling fee included). They are subject to change without notice. We reserve the right to adjust pricing errors and to limit quantities.

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