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BECKMAN COULTER

SDS-MW CE Kit - Protein Purity Analysis

The CE SDS-gel application has become the gold standard for protein purity analysis in biopharmaceutical laboratories. Denatured proteins can be reduced or left intact for separation and subsequent analysis.
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Automated, Quantitative Protein Purity Analysis with SDS-Gel Capillary ElectrophoresisSDS-MW Analysis Size Standard Electropherogram

 

 

 

 

 

 

TheA 800 plus Pharmaceutical Analysis System automates size separation of proteins and provides high-resolution, quantitative data, as compared to traditional labor-intensive slab gels.

The CE SDS-gel application has become the gold standard for protein purity analysis in biopharmaceutical laboratories. Denatured proteins can be reduced or left intact for separation and subsequent analysis.

Beckman Coulter's replaceable SDS-gel consists of a polymer matrix that allows for:

  • Quantitative and automated separation of proteins from 10-225 kD
  • Sensitivity equivalent to silver-stained gels when using LIF detection*
  • High-resolution separation capability

 PA 800 plus System Application Guides   PA 800 plus SDS-MW Application Guide

The following describes the IgG Purity and Heterogeneity method in detail:

Assay of IgG Purity and Heterogeneity Using High-Resolution Sodium Dodecyl Sulfate Capillary Gel Electrophoresis (AIB A-1973A) - This bulletin describes development of a replaceable SDS-gel formulation and a separation methodology for purity and heterogeneity assessment of IgG and its isoforms.


SDS-MW Analysis

High Resolution Protein Separation

Beckman Coulter's Automated SDS-MW Analysis Chemistries is designed for the separation and sizing of protein-SDS complexes using a replaceable gel matrix. The gel is formulated to provide an effective protein sieving range of approximately 10 kDa to 225 kDa. Within this size range, the logarithm of protein molecular mass is linear with its reciprocal electrophoretic mobility, allowing the molecular weight of an unknown protein to be estimated from a standard curve of known protein sizes.

This chemistry can also be used to effectively quantify the amount of protein and to determine the purity of a protein product.

SDS-MW Analysis Chemistries Includes:

  • Separation Capillary, 57 cm x 50µm ID bare fused silica, 2 pieces
  • SDS-Gel Separation Buffer, 140 mL
  • SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL
  • SDS Protein Sizing Standard (10 to 225 kDa) 16 mg/mL, 100 µL
  • Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL
  • Acidic Wash Solution, 0.1 N HCl, 100 mL
  • Basic Wash Solution, 0.1 N NaOH, 100 mL
  • SDS-Gel MW Analysis Guide, 1 piece

Click here for the SDS-MW Analysis Kit Insert.

Additional Information

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BECKMAN COULTER website
BECKMAN COULTER
Solutions and systems for clinical diagnostics (hematology, biochemistry, immunoassays, flow cytometry. Solutions for industry & research, for genetics, proteomics and cellular analysis. Lab automated workstation & liquid handling workstation.
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Prices are quoted in euro (taxes excluded) and apply in the Benelux (recycling fee included). They are subject to change without notice. We reserve the right to adjust pricing errors and to limit quantities.

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