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DESCRIPTION
Automated, Quantitative Protein Purity Analysis with SDS-Gel Capillary Electrophoresis
TheA 800 plus Pharmaceutical Analysis System automates size separation of proteins and provides high-resolution, quantitative data, as compared to traditional labor-intensive slab gels. The CE SDS-gel application has become the gold standard for protein purity analysis in biopharmaceutical laboratories. Denatured proteins can be reduced or left intact for separation and subsequent analysis. Beckman Coulter's replaceable SDS-gel consists of a polymer matrix that allows for:
PA 800 plus System Application Guides PA 800 plus SDS-MW Application Guide The following describes the IgG Purity and Heterogeneity method in detail: Assay of IgG Purity and Heterogeneity Using High-Resolution Sodium Dodecyl Sulfate Capillary Gel Electrophoresis (AIB A-1973A) - This bulletin describes development of a replaceable SDS-gel formulation and a separation methodology for purity and heterogeneity assessment of IgG and its isoforms. SDS-MW Analysis High Resolution Protein Separation Beckman Coulter's Automated SDS-MW Analysis Chemistries is designed for the separation and sizing of protein-SDS complexes using a replaceable gel matrix. The gel is formulated to provide an effective protein sieving range of approximately 10 kDa to 225 kDa. Within this size range, the logarithm of protein molecular mass is linear with its reciprocal electrophoretic mobility, allowing the molecular weight of an unknown protein to be estimated from a standard curve of known protein sizes. This chemistry can also be used to effectively quantify the amount of protein and to determine the purity of a protein product. SDS-MW Analysis Chemistries Includes:
Click here for the SDS-MW Analysis Kit Insert. Additional Information LINK
BECKMAN COULTER website
Prices are quoted in euro (taxes excluded) and apply in the Benelux (recycling fee included). They are subject to change without notice. We reserve the right to adjust pricing errors and to limit quantities.
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