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MOLECULAR DEVICES

Single-Mode Microplate Readers with Luminescence Detection

Designed for maximum detection sensitivity, the SpectraMax® L Microplate Reader limits the crosstalk between samples with an internal aperture for use with standard 96- and 384-well microplates.
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lifescience@analis.be
09 243 77 12
QUOTATION
INFORMATION
lifescience@analis.be 09 243 77 12
DESCRIPTION MODELS SPECIFICATIONS APPLICATIONS SOFTWARE FEATURES
DESCRIPTION
Pave the pathway to your future with the SpectraMax® L Microplate Luminometer, which can help you in so many ways. Measure flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminsescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. When you're ready to scale up, the SpectraMax L Reader can give you up to a six-fold increase in throughput with multi-channel configuration upgrade options. SoftMax Pro GxP Software and its software validation package give you comprehensive documentation and extensive data sets in CD-ROM format so you'll be able to meet FDA 21 CFR Part 11 and GxP compliance requirements.

 


Luminescence detection allows for greater sensitivity when monitoring biological or chemical processes that occur in everyday life, which makes luminescence detection an important element in the study of biology and other disciplines. Luminescence assays offer low background and broad dynamic range for reporter gene assays and other applications requiring high sensitivity. The pharmaceutical and biotechnology sectors use luminescence microplate readers for interests ranging from drug discovery research to quality testing.

Some of the problems associated with luminescence detection are crosstalk from bright sample wells, the speed of flash assays, separating signals from two different types of light-emitting molecules, and the amount of time required for screening. Through the introduction of the SpectraMax® L Luminescence Microplate Reader, Molecular Devices solved many of those issues. Improvements included upgradable injectors for flash assays, BRET reading capabilities with multiple detectors with filters for wavelength separation, the addition of more detectors to allow simultaneous reading of the microplate on the same instrument for higher throughput, and an aperture system for reducing crosstalk.

Common applications for luminescence include luciferase-based gene expression assays and ELISAs, as well as cell viability and cytotoxicity assays.


SpectraMax L Luminescence Microplate Reader

High sensitivity glow and flash luminescence detection from an upgradeable platform, offering up to six times the throughput of conventional microplate readers.

Designed for maximum detection sensitivity, the SpectraMax® L Microplate Reader limits the crosstalk between samples with an internal aperture for use with standard 96- and 384-well microplates. In conjunction with ultra-fast photon counting technology, the SpectraMax L Reader delivers a superior signal-to-noise ratio for luminescence. Extended over 9 orders of magnitude, the dynamic range of simultaneous photon counting and analog detection allows for capturing luminescence from dim samples while helping to avoid saturation from brighter samples that is unmatched by other microplate readers.

The SpectraMax L Microplate Reader is an upgradeable platform that can be added to over time, available with one or more luminescence detectors, enabling higher throughput configurations compared to single detector microplate readers. Optional injectors offer the capability to perform fast flash assays in addition to the more stable glow assays. These next generation injectors have a significantly extended lifetime over traditional syringe injectors. The precise dual injectors and detection sensitivity make the SpectraMax L Microplate Reader ideal for widely used dual luciferase reporter gene assays and carries the Promega DLReady certification.

Additional filters can be user-installed for Bioluminescence Resonance Energy Transfer (BRET) assays on multiple-detector SpectraMax L Microplate Reader systems.

Table 1. Six times the throughput with the SpectraMax L Microplate Reader. Estimated times to read a typical dual luciferase assay with different configurations. Total time includes significant delay and settling times in addition to injection and read times.  

Number of Detectors

Minutes to Read a 96-Well Plate

Total Plates Read in 8 Hours

1

40

12

2

20

24

6

6.7

72

 

The SpectraMax L Microplate Reader is supplied with SoftMax® Pro Software,
Molecular Devices' industry leading all-in-one data acquisition and analysis software. Additionally, the SpectraMax L Microplate Reader can be seamlessly integrated with the StakMax® Mi

MODELS
SPECIFICATIONS
APPLICATIONS
SOFTWARE
FEATURES
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Prices are quoted in euro (taxes excluded) and apply in the Benelux (recycling fee included). They are subject to change without notice. We reserve the right to adjust pricing errors and to limit quantities.

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