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BECKMAN COULTER

Reagent - RNA Isolation

RNA Purification Higher Quality, Faster Throughput Based on Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based technology, Agencourt Reagents are ideal for automating on Biomek Lab Automation Workstations. The end result is faster throughput and higher quality data.
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 figure 1

            

FormaPure - Total RNA Extraction from FFPE Tissue
The Agencourt FormaPure system provides a simple, automation-friendly process for extracting total nucleic acid from Formalin-Fixed, Paraffin-Embedded (FFPE) tissue samples. By utilizing the Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based technology, the Agencourt FormaPure system does not require vacuum filtration, centrifugation, or organic solvents such as phenol or xylene. Researchers can go from paraffin digestion to total nucleic acid extraction in about 4 hours (without setup) for 96 samples in a multi-well format utilizing the Beckman Coulter Biomek NXP or FXP Span-8 workstation. Recoveries are consistently higher than competitive techniques.

Application: Total RNA or total nucleic acid isolation from FFPE tissue samples
Downstream Application Techniques: qRT-PCR, microarray

Kit Features
  • Consistent, superior yield and purity of RNA or total nucleic acid
  • No centrifugation, vacuum filtration, or solvents required
  • Supports automated or manual processing                                                         

figure 2

 

 

 

 

 

 

 

 

RNAdvance Blood - Total RNA Extraction from Blood

Built on Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based technology, the Agencourt RNAdvance Blood system is a ribonucleic acid (RNA) isolation process. It enables the life science community to purify high quality RNA from blood collected using PAXgene* tubes. The kit is formatted to process one 96-well plate in about 3.5 hours.

Application: Total RNA extraction from blood

Downstream Application Techniques: Gene expression via qRT-PCR and microarray analysis

Agencourt RNAdvance Blood Features
  • Produces high quality nucleic acid
  • Compatible with downstream gene expression analysis techniques such as qRT-PCR and microarray
  • Amenable to automated processing on the Beckman Coulter Biomek FXP/NXP Span-8 Laboratory Automation Workstation
  • No use of vacuum filtration or organic extraction

 figure 3

 

 

 

 

 

 

 

 

 

 

RNAdvance Cell v2 - RNA Extraction from Cultured Cells

The Agencourt RNAdvance Cell v2 kit is based on Solid Phase Reversible Immobilization (SPRI) paramagnetic bead technology, known for its ability to consistently deliver pure nucleic acids of the highest quality and yield. This system reliably delivers high RNA recovery and purity without the need for filtration or centrifugation. The Agencourt RNAdvance Cell v2 chemistry is an automation friendly process for isolating total RNA for utilization in microarray and real time qPCR applications

Application: RNA extraction from cultured cells
Downstream Application Techniques: qRT-PCR, Microarray Analysis

Kit Features
  • Extraction and purification of high quality total RNA from cultured cells in a 96-well format
  • Efficient removal of genomic DNA and other contaminants
  • No centrifugation or vacuum filtration required
  • Ideal for gene expression applications
  • Scalable: Supports manual processing and full automation methods on Beckman Coulter Biomek NXP and FXP laboratory automation workstations
  • Throughput: Two 96-well plates in approximately 2 hours and 15 minutes on the Beckman Coulter Biomek FXP

 

 

 figure 4

 

 

 

 

 

 

 

 

 

 

 

RNAdvance Tissue - Total RNA Extraction from Tissue

The Agencourt RNAdvance Tissue total RNA extraction technology in combination with Beckman Coulter automation offers researchers a true walk away solution for consistent recovery of high quality total RNA in a multi-well format. Utilizing Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based technology, the Agencourt RNAdvance Tissue system can extract total RNA from a wide variety of tissues without the hazards and waste removal issues of organic solvents. The Agencourt RNAdvance Tissue system produces high recovery of total RNA from soft, fibrous, and lipid-rich tissues.

Application: Total RNA Extraction From Tissue
Downstream Application Techniques: qRT-PCR, Microarray Analysis

Agencourt RNAdvance Tissue Features
  • Extraction and purification of high quality total RNA from many tissue types
  • Efficient removal of genomic DNA and other contaminants
  • No centrifugation or vacuum filtration required
  • No organic extraction steps
  • Supports automated as well as manual processing
  • Ability to process from eight to 96 samples on a Biomek NX Workstation with a Span-8 configuration
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SPRI TECHNOLOGY
BECKMAN COULTER website
BECKMAN COULTER
Solutions and systems for clinical diagnostics (hematology, biochemistry, immunoassays, flow cytometry. Solutions for industry & research, for genetics, proteomics and cellular analysis. Lab automated workstation & liquid handling workstation.
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Prices are quoted in euro (taxes excluded) and apply in the Benelux (recycling fee included). They are subject to change without notice. We reserve the right to adjust pricing errors and to limit quantities.

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