Analis only distributes its products in Belgium and Luxembourg (some of our products also in the Netherlands). For product & price information for other countries, please contact our local suppliers (see attached list of suppliers & their websites) However, our IVD diagnostic kits are distributed all over the world. Please, contact us for information about all respective countries.
SEND ME A QUOTATION
Analis only distributes its products in Belgium and Luxembourg (some of our products also in the Netherlands). For product & price information for other countries, please contact our local suppliers (see attached list of suppliers & their websites) - However, our IVD Diagnostic kits are distributed all over the world. Please, contact us for information about all respective countries.
BECKMAN COUTLER - Complete solutions for Exosome Workflow
Look to Beckman Coulter for robust and streamlined methods designed to enable your exosomes research Exosome-based research is rapidly evolving in many fields of study given the growing understanding that these microvesicles exert influence through the delivery of mRNA, microRNA and protein. From RNA/protein isolation and analysis to sequencing, Beckman Coulter provides reliable lab instrumentation and process solutions to enable your research. Explore the various resources and application notes to learn more about how Beckman Coulter instrumentation is helping to advance research with isolation and purification method development
Contact our specialists Vincent JADIN FR Ann DESPLANQUES NL
What are exosomes and how are they isolated?
Published on Aug 9, 2016
A Path to the Standardiation of Exosome Isolation and NGS Characterization for Complex Disease Studies with Dr. Chad Schwartz.
Published on Sep 15, 2016
Traditionally, exosome isolation has been tedious, time-consuming, and subject to experimental variability. Ultracentrifugation is the gold standard for the isolation and purification of exosomes from biological fluids and cell culture samples, and enables accurate and reproducible yield of exosomes, while minimizing co-purification of protein aggregates and other membranous particles. To learn more about exosome isolation, please visit www.beckman.com/applications/exosomes
Testimonials and webinars:
Sep 15, 2016 In this video, Dr. Vannberg discusses his laboratory's research on the role of exosomes in disease. Learn how Dr. Vannberg's lab purifies exosomes for their research. To learn more, visit: www.beckman.com/applications/exosomes
May 11th, 2015
Isolation and Characterization of Exosomes and Ectosomes
Presented By: Dr. Suresh Mathivanan Laboratory head, Dept. of Biochemistry and Genetics, La Trobe Institute for Molecular Science (LIMS), La Trobe University
An improved and more efficient isolation and characterization protocol for exosomes and other extracellular vesicles (EVs) is critical to advancing this exciting field, and experts have recently called for the establishment of standardized methods. Ultracentrifugation is the gold standard1 for the isolation and purification of exosomes from biological fluids and cell culture samples, and enables accurate and reproducible yield of exosomes, while minimizing co-purification of protein aggregates and other membranous particles. Downstream challenges involve a standardized method for genetic profiling of encapsulated miRNA. The following white papers offer a look at using Biomek methods for centrifugation layering and fractionation, total RNA extraction, and cDNA amplification and clean-up for next generation sequencing. Recent studies have suggested that exosomes can serve as biomarkers for future clinical and diagnostic use in not only cancer, but many other human diseases2. Exciting new findings have implicated exosomes in cardiovascular diseases3-5, autoimmune syndromes6, and neurodegenerative disorders such as Alzheimer’s7 and Parkinson’s8 disease, in addition to infectious diseases such as tuberculosis9, diphtheria10, and even HIV11, and standardization of processes is an essential step for reliable and interpretable results.
A Standardized, Automated Approach for Exosome Isolation and Characterization Using Beckman Coulter Instrumentation This white paper details the protocol for exosome isolation and sequencing of exosome-derived RNA. LEARN MORE CLICK HERE
Exosome-Depleted FBS Using Beckman Coulter Centrifugation This white paper details the importance of exosome depletion of media and additives for use in exosome isolation studies. LEARN MORE CLICK HERE
g-Max: Added Capabilities to Beckman Coulter’s Versatile Ultracentrifuge Line This white paper details Beckman Coulter’s g-Max System, and how it can save you precious time during your exosome isolation procedure
Momen-Heravi, F., Balaj, L., Alian, S., Mantel, P-Y., Halleck, A.E., Trachtenberg, A.J., et. al. Current methods for the isolation of extracellular vesicles. Biol. Chem. 2013. Doi: 10.1515/hsz-2013-0141.
De Toro, J., Herschlik, L., Waldner, C., Mongini, C.. Emerging roles of exosomes in normal and pathological conditions: new insights for diagnosis and therapeutic applications. Front. Immunol. 2015. Doi: 10.3389/fimmu.2015.00203.
Amabile, N., Rautou, P-E, Tedgui, A., Boulanger, C.M.. Microparticles: key protagonists in cardiovascular disorders. Semin Thromb. Hemost. (2010) 36:907–16. doi:10.1055/s-0030-1267044Asdf.
DeJong O.G., Verhaar, M.C., Chen, Y., Vader, P., Gremmels, H., Posthuma, G., et. al. Cellular stress conditions are reflected in the protein and RNA content of endothelial cell-derived exosomes. J Extracell. Vesicles (2012) 1:18396. doi:10.3402/jev.v1i0.18396.
Waldenström, A., Gennebäck, N., Hellman, U., Ronquist, G.. Cardiomyocyte microvesicles contain DNA/RNA and convey biological messages to target cells. PLoS One (2012) 7:e34653. doi:10.1371/journal.pone.0034653.
Robbins, P.D., Morelli, A.E.. Regulation of immune responses by extracellular vesicles. Nat Rev Immunol (2014) 14:195–208. doi:10.1038/nri3622.
Rajendran, L., Honsho,M., Zahn,T.R., Keller,P., Geiger,K.D., Verkade,P., et. al. Alzheimer’s disease beta-amyloid peptides are released in association with exosomes. Proc. Natl. Acad. Sci. USA (2006) 103:11172–7. Doi:10.1073/pnas.0603838103.
Kruh-Garcia, N.A., Wolfe, L.M., Chaisson, L.H.,Worodria, W.O., Nahid P., Schorey J.S., et. al. Detection of Mycobacterium tuberculosis peptides in the exosomes of patients with active and latent M. tuberculosis infection using MRM-MS. PLoS One (2014) 9:e103811. doi:10.1371/journal.pone.0103811.
Colino, J., Snapper, C.M. Exosomes from bone marrow dendritic cells pulsed with diphtheria toxoid preferentially induce type1 antigen-specific IgG responses in naïve recipients in the absence of free antigen. J Immunol (2006) 177:3757–62. doi:10.4049/jimmunol.177.6.3757.
Gould S.J., Booth, A.M., Hildreth,J.E.K.. The Trojan exosome hypothesis. Proc Natl Acad Sci USA (2003) 100:10592–7. doi:10.1073/pnas.1831413100.
Prices are quoted in euro (taxes excluded) and apply in the Benelux (recycling fee included). They are subject to change without notice. We reserve the right to adjust pricing errors and to limit quantities.