Includes IgG Control Standard 1-pack and all chemistries.
The IgG Purity/Heterogeneity Assay has been developed for researchers employed in industrial biotechnology who are developing and manufacturing IgG reagents for research, diagnostic and therapeutic use.
This assay has been specified for use on the PA 800 Plus to assess the purity and heterogeneity of IgG reagents in both a reduced and non-reduced state. The methodology involves heat-denaturing a specified concentration of IgG (both reduced and non-reduced) in the presence of SDS, and separating these proteins by size using high-resolution capillary gel electrophoresis technology. This assay will detect impurities as low as 0.1% and includes an IgG control with a designated quantity of non-glycosylated heavy chain to test both the resolution and quantitation suitability of the assay prior to running unknowns
Assay chemistry includes:
• Separation Capillary, 57 cm x 50 µm ID bare fused-silica, (2)
• SDS Gel Separation Buffer (proprietary formulation), 140 mL
• SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL
• IgG Control Standard, 1 mg/mL in SDS sample buffer , 1 mL
• Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL
• Acidic Wash Solution, 0.1 N HCl, 100 mL
• Basic Wash Solution, 0.1 N NaOH, 100 mL
• IgG Purity and Heterogeneity Analysis Guide, (1)
LIF Test Mix, Sodium Fluorescein (20 mL). Test mix for testing the Laser-Induced Fluorescence (LIF) detector and determining the calibration correction factor (CCF) for the system.
High-efficiency particulate air (HEPA) filters remove particles smaller than 0.3 µm. Filters to remove volatile organic compounds (VOC – large group of carbon-based chemicals that easily evaporate at room temperature), which could potentially jeopardize embryo culture procedures. It has been known that the air quality in IVF laboratory can have higher levels of VOCs than outdoor environments air. The impact of VOC on embryo culture will significantly affect on embryo cleavage rate, embryo development and blastocyst formation rate.
ESCO HEPA filter with efficiency of 99.99% at 0.3 microns.
ESCO VOC filter to remove any compounds that are harmful to embryo development.
To be replaced every 3 months
Accessory for:
-MRI-6A10
-MRI-TL
Molecular Weight standards (10 to 225 KDa), 16mg/mL (100 µL) for use with the SDS-MW Kit (Part Number 390953) to provide a sizing ladder to reference proteins of interest against.
Polypropylene Co-Polymer (PPCO) bottle, 500 ml
(effective 435 ml), with screw cap, Ø 69 x 165 mm
For use in Sigma high-capacity centrifuges. Ask our experts for best centrifuge, rotors and adapter configuration.
No more need to mount capillaries. Pre-assembled EZ-CE cartridge (part # A55625) for multiple applications (Purity Analysis, Rapid Charge Variant Analysis, and Fast Glycan Analysis)
> Precision cut tips – ensure uniformity of injection plug from capillary to capillary
> Alignment precision at window – reduces potential failure due to misalignment
> Reduce preparation time for operator – simplifying the process and training requirements
Capillaries must be trimmed to optimize speed/resolution.
This capillary is used with the Carbohydrate Labeling and Analysis Kit.
Reorder component for P/N 477600.
eCAP™ Neutral Coated Capillary with laser burned window - 50 µm ID x 67 cm total length.
Capillary used with the Advanced cIEF Starter Kit (Part number A80976).
Capillaries must be trimmed to optimize for speed/resolution.
This capillary is also suitable for other applications where no EOF is desired. Thanks to the polyacrylamide coating, no EOF is present. The capillary can be used with both UV and LIF detection systems but capillary life is impacted when used with diodearray detection systems.
Quartz sample boats to be used with Milestone DMA-80 evo direct mercury analyser
Sensor cassette for Vi-CELL MetaFLEX Bioanalyze platform.
SC300 measures pH, pO2, pCO2, Glucose, Lactate, Electrolytes in cell culture medium and also more parameters associated with whole blood analysis
The SDS-Gel MW Analysis Kit is designed for the separation and sizing of protein–SDS complexes using a replaceable gel matrix. The gel is formulated to provide an effective protein sieving range of approximately 10 to 225 kDa. Within this size range, the logarithm of protein molecular mass is linear with its reciprocal electrophoretic mobility, allowing the molecular weight of an unknown protein to be estimated from a standard curve of known protein sizes. This chemistry can also be used to effectively quantify the amount of protein and to determine the purity of a protein product.
This includes:
• Separation Capillary, 57 cm x 50 µm ID, bare fused-silica, (2)
• SDS Gel Separation Buffer, 140 mL
• SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL
• SDS Protein Sizing Standard (10 to 225 kDa), 16 mg/mL, 100 µL
• Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL
• Acidic Wash Solution, 0.1 N HCl, 100 mL
• Basic Wash Solution, 0.1 N NaOH, 100 mL
• SDS-Gel MW Analysis Guide (1)
SDS-MW Gel Buffer is a 4-pack of the SDS-MW gel used in the SDS-MW Kit (Part Number 390953) and the IgG Purity and Heterogeneity Kit Assay (Part Number A10663).
SDS-MW gel designed for the separation and sizing of protein samples, utilizing SDS complexes as a replaceable gel matrix.